Kune IVF ( in vitro fertilization ), pane kazhinji kudiwa kuchengetedza masero ejema kana embryos. Kune miviri miviri mikuru ye cryopreservation ye embryos: inononoka kupisa uye kuwedzera.
Mhando dze cryopreservation ye embryos
Kupisa kwemazuva mashoma inzira inopfuura nguva, umo nzira yekuisa mvura inonaya kubva mumucheche uchishandisa mvura inonzi nitrogen inoshandiswa. Mukati iyi embryo ne-cryoprotective media (kudzivirirwa kubva kukuvadzwa nehutonho) inowanikwa muchero wepurasitiki uye yakanatswa kusvika ku 0,5 digiti paminiti kusvika kune -7 degrees. Zvadaro vanobatanidza uswa newaini inoputika yakakanganiswa mumvura inonzi nitrogen (inotonhora mvura kubva mumumvuyo), inononoka zvishoma nezvishoma kusvika kune -35 degrees, ndokuzoendeswa kune imwe yero nitrogen uye kunopisa zvakakwana kusvika kune -196 degrees.
Ikusavhiringidza kwenzira iri munzira iyo pachayo ndeyokuti, kune rumwe rutivi, kupera simba kwemvura kunobatsira kuponesa embryo pamusoro pemhepo inopisa, uye kune rumwe rutivi inogona kuiparadza zvachose nekuda kwekuyerera mvura - mvura inosanganiswa nemaproteine inobvawo mumuviri, iyo inoparadza masero.
Imwe nzira yemazuva ano ndiyo vitrification yemamirosi. Panguva imwecheteyo, kunonoka kupisa kunobviswa nekuumbwa kwemakristani echando. Tsvina yakagadzirwa nepurasitiki ine yakatendeka uye yakakomberedzwa inonzi cryoprotective media inokurumidza kuiswa mumvura ine nitrogen, iko kushanduka nekukurumidzira kwemvura yese kusvika kune mavreous state. Nenzira iyi, hapana kuora kwemvura kwembryo uye inongobvumira nyore kusingagadziki pasina kukuvadza.
Nenguva inononoka, kufa kwemazamu kunogona kubva kubva pa25 kusvika ku65%, uye kana pane vitrification - chete 10-12%. Mune nitrogen yemvura, embryo inogona kuchengetwa kwemakore gumi nemaviri. Mazai anotsvaga haafaniri nguva dzose anodiwa: anowanzodiridza mazai akawanda, asi haasi ma 2-3 embryo anoiswa mubereki yekuiswa. Asi mazai echando anogona kushandiswa nenguva, sezvisizvo nguva dzose mushure mekunge IVF pamuviri pakarepo inosvika, uye kusachengeta embryo kunoda kuedza kwekutevera.
Vitrification mazai uye spermatozoa
Mukuwedzera kune mazai anotsvaira, zvinogona kudikanwa kupisa uye majeremusi masero. Vitrification yehuta inogona kudiwa mushure mekuvhiyiwa kwemurume, mushure mokunge kukwanisa kwake kudiridza kunogona kuderedzwa. Pamberi pekuvhenekera, chirume chinotariswa uye chimwe chete chinoshandiswa chinoshandisa spermatozoa nekufamba zvakanaka uye pasina kukanganisa.