Iyi nzira, pasinei nekupararira kwayo, ine zvimwe zvinetso. Ndicho chinhu chinoita kuti vanyori vatsvake nzira itsva dzinobvumira kuchengeta ejaculate kwenguva yakareba. Iko kusakosha kukuru kweyo nzira yegyopreservation inotaurwa pamusoro apa inogona kunzi ichokwadi yokuti pashure pokupedza zvakakwana kwehurosi, kufamba kwemasero epabonde ari mukati maro kunoderera kusvika anenge 20-25%. Izvi zvinoreva kuti mukana wekuita pamuviri panguva yekudyarwa kwezai rakakura rine spermatozoa yakadaro inoderedzawo.
Kana uchitora semeni nenzira iyi, kuchengetedza mbeu inotora kutonhora kwe -196 degrees.
Nzira yekuchengetedza mbeu yekemikari nekombiyuta K. Saito
Iyi nzira yekuponesa varume ejaculate inogona kushandiswa mumatambudziko iwayo apo pasina chikonzero chekumirira kwenguva refu yeVFF nzira. Icho hachirevi kusvibiswa kwemunhu.
Paunenge uchishandisa nzira iyi, mbeu yekuchengetedza inoshandisa kushandiswa kwezvinonzi "electrolyte-free-medium" (BES). Sezvo zvakadaro, kusina munyu, mhinduro yakasimba yeglucose inowanzoshandiswa. Zvakakosha kuziva kuti kusvika pakuguma kwemaitiro ekuchengetedza hupenyu hwemadzimai ezvirwere zvepabonde mune imwe sarudzo hauna kudzidza. Zvisinei, zvidzidzo zvakawanda zvakaratidza kuti cation sodium uye potassium zvakachengetwa muchando, izvo zvisingabatanidzwi apo isotonic glucose inoshandiswa. Mumashoko akajeka, kushandiswa kweizvi sarudzo yekuchengetedza varume ejaculate kunobvumira kuchengetedza spermatozoa pasina kusviba, pasina kushandura marphological features.
Nzira iyi haibvumi kuchengetedza chirume chemazuva kwenguva yakareba sepo panguva yekudzivirira. Ndicho chikonzero icho chinogona kushandiswa, somuenzaniso, paunotora biomaterials mazuva mashomanana musati waVIV kana apo inotevera hurairi inotarisirwa kana iya yapfuura yakakundikana.
Muto wemvura inogona kuchengetwa sei?
Rudzi urwu rwemibvunzo inonyanya kufarira kune varume avo vasina kugadzirira panguva ino kuva baba.
Chokutanga pane zvose, zvinofanira kuonekwa kuti zvinhu zvose zvinoenderana nekusarudza nzira yekuchengetedza murume ejaculate. Munenge mese mapoka emvura aripo ikozvino anoshandisa nzira yekudyopreservation. Iko inokubvumira kuchengeta ejaculate kwenguva yakareba - kusvika kumakumi emakumi emakore.
Paunoshandisa nzira iyo isingasanganisi kupisa kwe ejaculate, inochengetwa kwete kupfuura mwedzi umwe. Sezvatotaurwa pamusoro apa, nzira iyi inoshandiswa kunyanya mukurongwa kwei in vitro fertilization.
Zvisinei, nguva shoma yakadai yekuchengetedza mbeu inenge isingasvibisi motility yepermatozoa.
Ejaculate inochengetwa sei?
Iyo ndiyo nzira yekusarudzwa kwehuta inoitwa mune imwe nzvimbo yepamusoro yezvokurapa. Mumwe murume anopiwa flask isina kuchena, iyo iyo ejaculate inounganirwa nekuita bonhora.
Mushure mokunge, bhorocha yacho pachayo inowanikwa mune imwe chigadziro chakakosha, iyo reagent iyo inonzi liquid nitrogen inowanzoonekwa, uye yakavharwa zvakasimba.
Pazvinenge zvichidiwa, chirume chinoputika chinobviswa uye chakasvibiswa. Zvadaro, hutano hwekuchengetedzwa kwayo hunoongororwa nekuongorora sampuli mune microscope inokosha.